Invasive procedure. Invasive methods of prenatal diagnosis of the fetus

Already by their name, invasive methods suggest a more serious nature of the indications for their implementation, since in themselves they are more traumatic and technically difficult to perform. And, most importantly, such methods are not always safe for the mother and fetus. On the other hand, the use of non-invasive methods is ubiquitous in the study of the health status of the expectant mother and child.

Invasive diagnostic methods during pregnancy

Amnioscopy - this invasive method during pregnancy is based on an assessment of the quantity and quality of amniotic fluid. Its implementation involves the introduction of a special device (endoscope) into the cervical canal, and through a visual assessment of the above data, a conclusion is made. A decrease in the amount of water and the detection of meconium elements in them are unfavorable diagnostic signs in assessing the further condition of the fetus. The technique for performing invasive methods during pregnancy is not too complicated. However, amnioscopy is only possible if the cervical canal can "miss" the instrument. This examination is technically possible at the end of pregnancy, when the cervix is ​​being prepared for childbirth and the cervical canal is partially opened.

Amniocentesis is a puncture of the amniotic cavity to collect amniotic fluid. Carrying out this diagnostic method during pregnancy is possible with the help of transabdominal access under ultrasound control of the manipulation being performed. The puncture is performed in the area of ​​the largest "pocket" of amniotic fluid, where there are no parts of the fetus and umbilical cord loops, avoiding possible trauma to the placenta. 10–20 ml of amniotic fluid are aspirated, depending on the goals of diagnosis. As a rule, this research method is used to diagnose congenital and hereditary diseases of the fetus, to more accurately diagnose the maturity of the lungs of the fetus.

Cordocentesis is the puncture of the vessels of the umbilical cord of the fetus in order to obtain its blood. This method is performed transabdominally under ultrasound guidance. Manipulation is carried out in the second and third trimesters of pregnancy. This invasive method is used both for the diagnostic purpose of various diseases of the fetus, and for therapeutic purposes.

Chorionic biopsy (chorionbiopsy) - obtaining chorionic villi and their further detailed study. The implementation of this invasive method is diverse. Currently, aspiration transcervical or transabdominal puncture chorionbiopsy is most often used in the first trimester of pregnancy. The sampling (aspiration) of the material (chorion) for research is carried out under the control of ultrasound scanning using a special catheter or a puncture needle inserted into the thickness of the chorion. The main indication for this diagnostic method is the prenatal diagnosis of congenital and hereditary diseases of the fetus.

Aspiration of fetal urine is advisable in obstructive conditions of the urinary system. It is performed by puncture of the bladder or renal pelvis of the fetus under ultrasound guidance. The urine obtained in this case is subjected to an extended biochemical study to assess the functional state of the renal parenchyma and clarify the question of the need for antenatal surgical correction.

Fetal skin biopsy is an invasive diagnostic method based on obtaining fetal skin by aspiration or forceps under ultrasound control or fetoscopic control for the purpose of prenatal diagnosis of hyperkeratosis, ichthyosis, albinism and other diseases (mainly skin and connective tissue).

A tissue biopsy of tumor-like formations is performed by aspiration sampling of tissues of a solid structure or the contents of cystic formations in order to diagnose and select tactics for managing this pregnancy.

Liver tissue biopsy - obtaining samples of fetal liver tissue by the same aspiration method for the diagnosis of diseases during pregnancy associated with a deficiency of specific liver enzymes.

Amnioscopy as a method of diagnosing pregnancy

Amnioscopy is a method that allows you to examine the fetal membranes and amniotic fluid, which are visible through intact membranes (amnion and smooth chorion) adjacent to the internal os. Carrying out amnioscopy at a later date is possible due to the fact that you can enter the amnioscope without any difficulty.

The amnioscope is a special device equipped with a lighting fixture that allows you to visually evaluate the condition of the fetal membranes and amniotic fluid.

In the normal course of pregnancy, the nature of the waters is as follows: transparent or slightly cloudy (due to the admixture of cheese-like lubricant, epidermis and vellus hair). If green waters are determined, we can talk about the presence of hypoxia or fetal asphyxia. This situation is possible with a number of pathologies (the presence of preeclampsia of the second half, an infectious disease with a rise in temperature during pregnancy, etc.). Amnioscopy also allows you to clarify the presence of polyhydramnios, premature rupture of amniotic fluid and identify changes that occur during intrauterine death of the fetus.

Non-invasive diagnostic method in pregnant women - amniocentesis

The next method of diagnosing pregnancy is amniocentesis. This method is performed by puncturing the membranes and extracting a small amount of water for research. This method is performed strictly according to the indications (suspicion of severe hemolytic fetal jaundice, severe asphyxia, etc.).

In the future, a biochemical study of the obtained waters allows you to accurately establish the diagnosis. It should be noted that it is possible to examine the obtained waters for genetic composition, which makes it possible to determine the sex of the fetus (by the content of sex chromatin), the presence of chromosomal aberrations (anomalies).

Non-invasive diagnostic methods during pregnancy

After examining the external genital organs and the mucous membrane of the entrance to the vagina, pregnancy is examined using mirrors. It is with the help of this non-invasive diagnostic method during pregnancy that it is possible to detect the presence of cyanosis of the cervix and vaginal mucosa (a likely sign of pregnancy), as well as to detect diseases of the cervix and vagina, such as

  • inflammatory process,
  • cervical erosion,
  • polyp,
  • cancer, etc.

Used in the study of pregnancy with the help of mirrors, folding and spoon-shaped mirrors. The folded speculum must be inserted up to the vault of the vagina in a closed form, and then the folds are opened, and the cervix becomes available for inspection. The walls of the vagina are examined with the gradual removal of the mirrors from the vagina at the end of the study.

Sufficiently good access for examining the cervix of the vagina is created using spoon-shaped mirrors. First, the posterior mirror is inserted, placed on the back wall of the vagina and slightly pressed on the perineum. Further, parallel to it, an anterior mirror (flat lifter) is inserted, with which the anterior wall of the vagina is raised. After examining the cervix and vaginal vaults, the mirrors are removed and manual vaginal examination is started.

Bimanual research method during pregnancy

Having finished feeling the cervix, proceed to a two-handed study. The fingers inserted into the vagina are placed in its anterior fornix, the cervix is ​​slightly pushed back. With the fingers of the left hand, gently press on the abdominal wall towards the cavity of the small pelvis, towards the fingers of the right hand, located in the anterior fornix. Bringing together the fingers of both investigating hands, find the body of the uterus and determine its position, shape, size, consistency. Next, a study of the fallopian tubes and ovaries is performed. To do this, the fingers of the inner and outer hands are gradually moved from the corners of the uterus to the side walls of the pelvis. The diagnosis of pregnancy is completed with the help of mirrors by probing the inner surface of the pelvic bones:

  • inner surface of the sacrum,
  • side walls of the pelvis
  • and symphysis, if available.

They find out the approximate capacity and shape of the pelvis, try to reach the cape, measure the diagonal conjugate.

Bimanual vaginal examination of pregnancy using mirrors determines the following signs.

An increase in the size of the uterus, which becomes noticeable already at the 5-6th week of pregnancy. An increase in the uterus is initially noted in the anteroposterior size (becomes spherical), while later its transverse size also increases. The longer the gestation period, the clearer the increase in uterine volume. By the end of the second month of pregnancy, the uterus increases to the size of a goose egg, at the end of the third month of pregnancy, the bottom of the uterus is at the level of the symphysis or slightly above it.

The sign of Horvitz-Hegar is characterized by the fact that the consistency of the pregnant uterus is soft, and the softening is especially pronounced in the isthmus. As a result, with the two-handed vaginal diagnostic method, the fingers of both hands meet in the isthmus almost without resistance. This symptom is most characteristic of early pregnancy.

Piskacek's sign is characterized by the appearance of uterine asymmetry in early pregnancy. This is manifested by the appearance of a dome-shaped protrusion of the right and left corners of the uterus. The place of protrusion corresponds to the place of implantation of the fetal egg. In the future, as the fetal egg grows, the protrusion disappears.

The sign of Snegireva is characterized by a change in the consistency of the uterus during pregnancy. The softened pregnant uterus during a two-handed examination under the influence of mechanical irritation becomes denser and shrinks in size. After the cessation of irritation, the uterus again acquires a soft texture.

Genter's sign is characterized by the occurrence of an anterior inflection of the uterus in the early stages of pregnancy as a result of a strong softening of the isthmus, as well as the appearance of a ridge-like thickening (protrusion) on the anterior surface of the uterus along the midline. The appearance of thickening, however, cannot always be determined.

The Gubarev-Gauss sign is characterized by the appearance of slight mobility of the cervix. In early pregnancy, slight displacement of the cervix is ​​associated with significant softening of the isthmus.

The presence of the above signs in combination with probable signs makes it possible to assume or accurately diagnose pregnancy. If there is any doubt about the identification of signs, the woman should be asked to return for a follow-up examination in 1–2 weeks. During this time, the uterus increases in size, and all signs become apparent.

Rectal examination in diagnostics in pregnant women

In pregnant women in the second half of pregnancy, rectal examination should also not be neglected.

The study is carried out with gloves. Lubricated with vaseline, the second finger is inserted into the rectum and the neck, presenting part, identification points, pelvic walls are felt.

Outside of the contraction, a slow downward pressure is made towards the descending head. As a result, the location of the head can be determined. So, if the head is located in the outlet or in the narrow part of the pelvic cavity, it is determined quite easily, and if it is in the wide part, it is difficult.

Genter's method gives an idea of

  • degree of smoothing of the neck and opening of the pharynx,
  • the condition of the fetal bladder (if it is intact and tense), the presenting part and identification points,
  • as well as the relation of the head (or buttocks) to one or another plane of the pelvis.

Biological methods for diagnosing pregnancy

Also in this situation, resort to biological methods for diagnosing pregnancy. This non-invasive diagnosis is also essential in recognizing some types of pathological pregnancies. For example, it is not always easy to distinguish an ectopic pregnancy from inflammation of the uterine appendages, it is often difficult to differentiate pregnancy from a uterine tumor, etc.

The first moment of the biological method for diagnosing pregnancy is to establish the presence of human chorionic gonadotropin in the urine. This can be done using the Ashheim-Zondex or Friedman reaction, the implementation of which involves the injection of the urine of a pregnant woman subcutaneously into immature mice, which leads to the growth of the uterus and ovarian follicles in these animals, as well as the appearance of hemorrhages in the cavity of enlarged follicles. However, these methods are practically not used in modern obstetrics. The Friedman reaction also implies the introduction of pregnant urine only to rabbits.

Also, the presence of chorionic gonadotropin in the urine of a woman is practically not used at the present time to diagnose pregnancy, a hormonal reaction in frogs. This reaction consists in the fact that the test urine, introduced into the dorsal lymphatic duct of a male frog, in the presence of a hormone in it, causes the release of spermatozoa after 1–2 hours.

You can also establish the presence of this hormone in the urine using special test systems that are easy to purchase at a pharmacy (pregnancy test). In this case, the woman herself performs the determination of the presence of the hormone in the urine.

Immunological non-invasive method for diagnosing pregnancy

To diagnose pregnancy, it is possible to use an immunological method based on the reaction between chorionic gonadotropin in the urine of pregnant women and antiserum. The most commonly used diagnostic method is based on inhibition of the hemagglutination reaction of erythrocytes treated with chorionic gonadotropin with an appropriate antiserum in the presence of chorionic gonadotropin contained in the urine of pregnant women.

Antiserum is obtained after immunization of rabbits. If the test urine contains chorionic gonadotropin, and, therefore, the woman whose urine is examined is pregnant, then the hemagglutination reaction will not occur (chorionic gonadotropin will bind antibodies).

Also, a modern method for diagnosing pregnancy for the presence of chorionic gonadotropin in the blood serum is the radioimmunological method, which makes it possible to determine the level of chorionic gonadotropin equal to 0.12–0.50 IU / ml in 5-7 days. There are even more modern radioimmunological methods that determine the beta chains in the chorionic gonadotropin molecule already at its level of 0.003 IU / ml. The implementation of these methods requires only 1.5–2.5 minutes.

Instrumental research methods in the second half of pregnancy

In the second half of pregnancy, instrumental research methods are also used.

The necessary research methods in the second half of pregnancy are phonocardiography and electrocardiography - methods aimed at listening and recording fetal heartbeats.

Phonocardiography allows you to identify low frequencies of oscillations emanating from the fetal heart, which are not captured by auscultation. This method accurately reflects the mode of fetal cardiac activity - acceleration, slowdown, arrhythmia, etc., which, in particular, is a diagnostic criterion for fetal hypoxia and asphyxia.

Electrocardiography allows you to fix the cardiac activity of the fetus from 14-16 weeks of pregnancy, which is impossible with phonocardiography.

It also provides important information ultrasound procedure. The ultrasound method allows you to determine

  • fruit size,
  • presenting part,
  • cord length,
  • her twist,
  • location of the placenta
  • nature of amniotic fluid, etc.

Invasive diagnostic methods (IMD) is a combined group of studies that allow obtaining biological material of fetal origin (amniotic fluid, chorionic or placental villi, skin areas and fetal blood) for analysis. This is an indispensable way to diagnose many hereditary diseases, metabolic diseases, immunodeficiency states, which often do not have pronounced signs, determined by other methods.

The choice of method is carried out jointly by a geneticist and an obstetrician-gynecologist, taking into account the gestational age and specific pathology. When choosing invasive procedures, the possibility of termination of pregnancy and the occurrence of other complications should always be taken into account. With each pregnancy, there is a so-called "basic risk" of fetal loss, which consists of a combination of a woman's diseases and exposure to environmental factors, and averages 2-3%. As the pregnancy progresses, this risk decreases.

Additionally When carrying out even the safest invasive method - amniocentesis, the probability of abortion increases by 0.2 - 2.1% and averages 2.5 - 5.2%. The frequency of fetal loss depends on the technical equipment of the clinic, the qualifications of the doctor, the research method and the general condition of the pregnant woman.

Dates

There are different classifications of invasive diagnostic methods.

According to the timing, there are:

  • IMD performed in the first trimester of pregnancy:
  1. chorionic villi - cells of the chorionic villi (the outer membrane of the fetus, which later transforms into the placenta) are taken for research to determine the chromosome set of the fetus. Samples are taken at 8-12 weeks of gestation.
  2. - an operation by which amniotic fluid is obtained for examination. The timing is the same as for a chorionic villus biopsy, but since there is a high risk of abortion, it is more often performed in the second trimester.
  • IMD performed in the second trimester of pregnancy:
  1. Amniocentesis Taking amniotic fluid is usually at 17 - 22 weeks of pregnancy, but sometimes the study is carried out up to 34 weeks.
  2. method of visual inspection of the lower pole of the fetal egg using a thin endoscope. It can be carried out from 17 weeks of pregnancy and, if necessary, up to childbirth.
  3. the procedure for taking placental cells for analysis for the diagnosis of chromosomal diseases. Carried out at 18 - 22 weeks.
  4. receipt for a fetal blood test for the diagnosis of hereditary blood diseases, intrauterine infection, as well as the treatment of fetal hemolytic disease. Apply from 18 weeks of pregnancy.
  5. direct examination of the fetus to detect congenital malformations. Using an endoscope, it is also possible to take a piece of fetal skin for examination. Usually carried out at 18 - 24 weeks.

important In the third trimester of pregnancy, as a rule, IMD is not used due to the high risk of preterm birth. But sometimes, in the presence of strict indications, it is possible to perform amnioscopy, amniocentesis and cordocentesis before childbirth.

Depending on the location of the placenta, the following types of access are distinguished :

  • Transabdominal - insertion of the instrument through the anterior abdominal wall;
  • Transcervical - enter the uterine cavity through the cervical canal;
  • Transvaginal - pierce the anterior or posterior fornix of the vagina.

Indications for IMD:

  • The age of a woman is over 35 years old, since the frequency of spontaneous mutations increases with age even in the absence of other risk factors;
  • The presence of signs of congenital pathology with;
  • Deviation of the level of serum proteins in the mother's blood;
  • consanguineous marriage;
  • The presence of a chromosomal rearrangement, a hereditary disease or a malformation in one of the spouses;
  • The birth of a child with a hereditary disease or malformation;
  • A history of spontaneous miscarriages, stillbirths, primary amenorrhea, primary infertility in spouses;
  • Adverse effects of environmental factors in early pregnancy (radioactive exposure, inhalation of vaporous poisons, etc.);
  • Taking embryotoxic drugs in early pregnancy;
  • X-ray examination in the early stages;
  • Group or Rh incompatibility of mother and fetus.

Contraindications:

  • The threat of termination of pregnancy;
  • Inflammatory diseases of the vagina and cervix, or the skin of the abdomen (depending on the puncture site).

Possible complications after IMD:

  • premature rupture of amniotic fluid,
  • fetal injury,
  • cord injury,
  • injury to the bladder and intestines of the mother,
  • chorioamnionitis (inflammation of the membranes).

All invasive methods of fetal diagnostics are carried out only with the consent of the pregnant woman. Before making a decision, it is necessary to weigh the pros and cons as calmly as possible and only then refuse to conduct the study. Very often, pregnant women do not understand that such procedures are not simply prescribed, and a serious disease of the fetus that is not detected in time can threaten not only the health, but also the life of a woman.

Invasive methods

These methods, already by name, suggest a more serious nature of the indications for their implementation, since in themselves they are more traumatic and technically difficult to perform and, most importantly, are not always safe for the mother and fetus.

1. Amnioscopy - this method is based on assessing the quantity and quality of amniotic fluid. Its implementation involves the introduction of a special device (endoscope) into the cervical canal, and through a visual assessment of the above data, a conclusion is made. A decrease in the amount of water and the detection of meconium elements in them are unfavorable diagnostic signs in assessing the further condition of the fetus. The execution method is not too complicated. However, amnioscopy is only possible if the cervical canal can "miss" the instrument. This examination is technically possible at the end of pregnancy, when the cervix is ​​being prepared for childbirth and the cervical canal is partially opened.

2. Amniocentesis - puncture of the amniotic cavity to collect amniotic fluid. Carrying out this research method is possible with the help of transabdominal access under ultrasound control of the manipulation. The puncture is performed in the area of ​​the largest "pocket" of amniotic fluid, where there are no parts of the fetus and umbilical cord loops, avoiding possible trauma to the placenta. 10–20 ml of amniotic fluid are aspirated, depending on the goals of diagnosis. As a rule, this research method is used to diagnose congenital and hereditary diseases of the fetus, to more accurately diagnose the maturity of the lungs of the fetus.

3. Cordocentesis - puncture of the vessels of the umbilical cord of the fetus in order to obtain its blood. This method is performed transabdominally under ultrasound guidance. Manipulation is carried out in the second and third trimesters of pregnancy. This method is used both for the diagnostic purpose of various diseases of the fetus, and for therapeutic purposes.

4. Chorionic biopsy (chorionbiopsy) - obtaining chorionic villi and their further detailed study. The implementation of the method is diverse. Currently, aspiration transcervical or transabdominal puncture chorionbiopsy is most often used in the first trimester of pregnancy. The sampling (aspiration) of the material (chorion) for research is carried out under the control of ultrasound scanning using a special catheter or a puncture needle inserted into the thickness of the chorion. The main indication for this diagnostic method is the prenatal diagnosis of congenital and hereditary diseases of the fetus.

Urine aspiration the fetus is appropriate for obstructive conditions of the urinary system. It is performed by puncture of the bladder or renal pelvis of the fetus under ultrasound guidance. The urine obtained in this case is subjected to an extended biochemical study to assess the functional state of the renal parenchyma and clarify the question of the need for antenatal surgical correction.

Fetal skin biopsy a diagnostic method based on obtaining fetal skin by aspiration or forceps under ultrasound control or fetoscopic control for the purpose of prenatal diagnosis of hyperkeratosis, ichthyosis, albinism and other diseases (mainly skin and connective tissue).

Biopsy of tumor tissue is performed by aspiration sampling of tissues of a solid structure or the contents of cystic formations in order to diagnose and select tactics for managing this pregnancy.

Biopsy of liver tissue- obtaining samples of fetal liver tissue by the same aspiration method for the diagnosis of diseases associated with a deficiency of specific liver enzymes.

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7.5.3. Surgical methods 7.5.3.1. Vital pulp extirpation Vital pulp extirpation (pulpectomy) is the most common method of pulpitis treatment in world practice. The indications for pulp extirpation are as follows:? any form of inflammation of the pulp;

Invasive from the Latin invasio - penetration, invasion, penetration.

Invasive research methods- these are methods based on the introduction of substances into the body cavity - intramuscularly, intravenously or with damage to the skin, mucous membranes.

Also, instrumental methods can be attributed to invasive methods - deep penetration into the body's natural pathways, for example, colonoscopy. Visualization of the internal structures of the body, obtaining tissue or cell samples for research (biopsy) is the main purpose of invasive methods. Their use may be accompanied by the risk of bleeding, infection, mechanical damage to organs.

In this regard, modern medicine tends to move towards replacing invasive methods with minimally invasive, non-invasive ones.

Invasive diagnostic methods are used in prenatal diagnostics - placentobiopsy, biopsy of fetal tissues, amniocentesis - obtaining amniotic fluid, cordocentesis - taking fetal blood; in the diagnosis of respiratory diseases - bronchoscopy, bronchography, pleural puncture, pleural biopsy, lung biopsy and others; as well as in cardiology, urology, gastroenterology.

Invasive diagnostic methods allow you to make a reliable diagnosis, they provide high diagnostic accuracy.

The information provided on the site is for informational purposes only. To make the correct diagnosis and choose the right treatment tactics, you need to seek help from a doctor.

Pleurisy: stages and diagnostic methods

Pleurisy - inflammation of the pleura sheets, at first glance, a simple disease is a clear sign of serious and even insidious diseases, like oncology. Because it refers mainly to secondary lesions, due to structural and functional disorders in the surrounding organs. It proceeds in the form of dry (fibrinous) and exudative (effusion) pleurisy.

Classification of pleurisy - causes

According to the causes, they are divided into:

pleurisy of infectious origin, resulting from the penetration of the pathogen between the sheets of the pleura (by contact, through the lymph and blood):

  • bacterial (tuberculosis, bacterial pneumonia (pneumonia), syphilis, brucellosis, tularemia)
  • viral and rickettsial (atypical viral pneumonia, psittacosis, Q fever)
  • fungal (coccidioidomycosis, blastomycosis, candidomycosis)
  • protozoan (amebiasis)
  • helminths (echinococcus, trematodes).

non-infectious pleurisy, due to pathological processes in other organs:

  • heart diseases
  • oncological processes
  • kidney dysfunction
  • diseases of the gastrointestinal tract (cirrhosis of the liver, pancreatitis, alimentary dystrophy)
  • autoimmune connective tissue diseases
  • blood pathology (thrombocytopenia, leukemia)
  • lung atelectasis
  • ovarian fibroma (Meigs syndrome)

Questioning and physical data - the basis of a correct diagnosis

The set of characteristic complaints of the patient is the main symptoms, they will differ in different forms of pleurisy:

Dry pleurisy is manifested by intense, stabbing, more often, one-sided pain in the area of ​​the affected lung, associated with a change in body position; when coughing, sneezing, inhaling as deeply as possible and moving in the opposite direction, it intensifies; lying on the sore side the pain decreases; shallow breathing. Perhaps fever, sweating, weakness, loss of appetite.

Exudative pleurisy is characterized by heaviness in the chest cavity, shortness of breath, cough, cyanosis. Pain is present in carcinomatous lesions of the pleura. Signs of purulent pleurisy are febrile (38 ° C and above) temperature, pain when breathing, symptoms of general malaise, severe sweating and chills.

The collection of an anamnesis of the disease is an important diagnostic step, due to the secondary nature of pleurisy. The doctor carefully asks the patient:

where the patient lives and works (to exclude infection with fungi, rickettsia and viruses in the course of personal and professional activities);

nutritional features (exclude protozoal infections and helminthiases);

about contacts with sick people (tuberculosis, syphilis) and past infectious diseases;

complaints and possible ailments from the cardiovascular system, gastrointestinal tract, kidneys, blood, ovaries (for women), connective tissue.

Physical findings include examination of the patient, palpation, percussion and auscultation.

In a patient with dry pleurisy, on examination, asymmetry of the chest during breathing is visible, and in a patient with exudative pleurisy, a decrease in chest excursion during breathing and expansion of the intercostal spaces on the affected side.

Dry pleurisy is detected by palpation behind the pleural friction noise and voice trembling disorders, pain in the trapezius and pectoral muscles is characteristic of the apical localization of dry pleurisy.

The sound during percussion (when tapping) may remain unchanged in a patient with dry pleurisy. Exudative pleurisy is manifested by a dull or dull sound, the upper line of which is called Sokolov-Ellis-Damuazo.

Auscultation (listening) of a patient with dry pleurisy reveals a pleural friction noise, which is the same on inhalation, exhalation and raising and lowering the chest without inhaling air, persists after coughing (as opposed to moist rales in pneumonia). Exudative pleurisy - weakened (above the site of effusion) and bronchial breathing (above the compressed lung tissue, above the exudate).

At the initial stage, after a detailed survey of the patient, taking into account the localization of pain (where does it hurt?), its irradiation (where does it spread?) and the data of the physical examination, the doctor forms an opinion about the form of pleurisy, its nature (infectious or non-infectious). This knowledge will help in the appointment of the following laboratory and instrumental methods of research and the correct diagnosis.

What laboratory tests are needed to diagnose pleurisy?

A general blood test for pleurisy of inflammatory origin is characterized by leukocytosis, a shift to the left of the leukocyte formula, sometimes anemia, an increase in ESR and monocytosis and eosinopenia inherent in tuberculous lesions.

In the analysis of urine, a small amount of protein can be determined, erythrocytes and epithelial cells are found.

A biochemical blood test reveals an increase in the level of sialic acids and fibrinogen in combination with a normal amount of total protein. There may be a decrease in albumin levels and an increase in globulins in the acute phase of the disease. Given the complaints and the patient's condition, determine the levels of glucose, cholesterol, rheumatic factor, liver tests.

With the possible tuberculous nature of pleurisy, a Mantoux test is prescribed - a specific intradermal test for sensitivity to Mycobacterium tuberculosis.

To exclude syphilis, a blood test is performed for RW (Wasserman reaction).

The results of laboratory tests help to determine the cause (etiological factor) of pleurisy and to choose the right instrumental research methods.

Diagnostic capabilities of non-invasive research methods

Using instrumental methods, the area (scale) of the lesion and the nature of the inflammatory process are determined.

Non-invasive research methods include X-ray, ultrasound, ECG, FVD.

  1. fluoroscopy - the most common and mandatory, shows the presence of fluid in the pleural cavity, according to the type of uniform darkening with a clear and oblique line from above. When there is little fluid, it is visually manifested by a thickening of the costal margin, in case of a massive lesion, the mediastinum moves to the healthy side. Provides an opportunity to see the movement of fluid with free pleural effusion due to a change in the position of the body and breathing of the patient. Dry pleurisy manifests itself: the diaphragmatic dome is high, lags behind at maximum inspiration, the lower borders of the lungs do not have adequate mobility, they are compacted.
  2. radiography - carried out in 2 projections: a survey in direct projection can miss the disease with a fluid volume of up to 300 ml. The laterogram (lying the patient on his side) allows you to determine the presence of effusion up to 100 ml and distinguish it from adhesions, previously transferred inflammatory processes.
  3. x-ray computed tomography - is characterized by high diagnostic value: it qualitatively determines the state of the lung tissue (parenchyma), mediastinum, pleural cavity and the pleura itself, already at the initial stage of the disease detects the presence of effusion. The use of contrast helps to identify encysted pleurisy with local effusions, differentiate with lung tissue damage, and determine the nature of pleural neoplasms. Knotty thickening of the pleura with diverging circles indicate the malignant nature of the changes.
  4. Bronchography is a contrast method for studying the cavity of the bronchi, used for the purpose of differential diagnosis to identify oncological processes in the bronchi.
  5. Ultrasound examination - allows you to identify a small amount of pleural fluid (5 ml), distinguish it from thickening and fibrosis of the pleura, detect a hidden diaphragmatic dome under the effusion, a fairly informative and convenient method for puncture, biopsy and drainage.
  6. ECG (electrocardiography) - in order to differentiate pleurisy on the left side and myocardial infarction, given the possible displacement of the mediastinal organs and the axis of the heart with massive pleurisy and adhesions.
  7. FVD (function of external respiration) - the vital capacity of the lungs (VC) is reduced due to restrictive violations of the pleura.

The above methods of non-invasive diagnostics have their advantages and disadvantages. Given them, you need to correctly use the capabilities of the method for different forms of pleurisy. So, fluoroscopy plays a major role in the diagnosis of exudative pleurisy. If necessary, it is necessary to combine the use of X-ray with other studies to improve the diagnostic accuracy of the method.

Invasive methods - a combination of diagnosis and treatment

Invasive diagnostic methods include pleural puncture thoracoscopy.

Pleural puncture: manipulation consists in puncturing the chest and pleura for the purpose of diagnosis and treatment. Before the procedure, the patient's morale and premedication (preparation for anesthesia) are carried out. During its implementation, the patient sits with his back to the doctor, hands on the table, in severe cases - it is allowed in the supine position. Under sterile conditions, observing the rules of asepsis, first disinfect the site of the proposed puncture with iodine and chlorhexidine and dry it with a napkin. The skin is anesthetized with a 0.5% novocaine solution. The syringe is connected to a thin puncture needle using a rubber tube with a clamp, it will not allow air to enter the pleural cavity. The doctor performs a puncture in the seventh-eighth intercostal space along the upper edge of the rib (excludes nerve damage) in order to remove the accumulated fluid. Fills the syringe slowly, transfers the effusion into a sterile dish for further research. The skin around the wound is compressed, disinfected and sealed. To prevent the development of complications, the patient stays under the supervision of medical staff for a day.

The resulting fluid is examined by studying biochemistry, cytology and flora.

Visually, one can distinguish a transparent yellowish transudate (non-inflammatory congestive effusion) and the following types of inflammatory exudate:

  • Serous - similar to transudate, transparent and odorless;
  • Purulent - thick, cloudy from grayish to yellow-green, mostly odorless, only fetid with gangrene;
  • Hemorrhagic - color from slightly pink to intense brown, which depends on the amount and duration of blood penetration into the pleural cavity, contains red blood cells (erythrocytes) with changes and unchanged structure, corresponds to oncological processes of the pleura and lungs, tuberculosis and traumatic lesions of the pleura, rarely pneumonia ;
  • Chylous - milky with a large amount of fat, associated with impaired lymph circulation in the thoracic duct due to neoplasms, enlarged lymph nodes, or its rupture;
  • Cholesterol - from rich yellow to brown, quite thick, indicates long-standing localized processes.

In a biochemical study:

  • in transudates, a small amount of protein is determined up to 25 g / l and, accordingly, the density is in the range of 1.002-1.015;
  • for exudates, a high level of protein ≥ 30 g / l (for purulent up to 70 g / l), a relative density of 1.015 and above, a positive Rivalt test is inherent;
  • the amount of glucose up to 3 mmol / l is determined in tuberculosis, malignant neoplasms, violation of the integrity of the esophagus, inflammation of the lungs, autoimmune diseases (rheumatoid arthritis, early stage of pleurisy in lupus);
  • high amylase levels occur in effusions due to pancreatitis, esophageal rupture, lung adenocarcinoma (rare);
  • rheumatoid (rheumatoid arthritis) and antinuclear factor (systemic lupus erythematosus) can be detected by immunological methods.

Microbiological (cytological) examination is carried out:

  • native (unstained) smears for studying the qualitative and quantitative composition of cells (erythrocytes, lymphocytes, tumor cells, fat drops, cholesterol, etc.);
  • stained smears to determine the percentage of individual types of lymphocytes, a detailed study of the structure of cells. The presence of eosinophils is characteristic of allergic processes in the lungs and pleura, and mesothelium is found in the initial stage of an inflamed reaction and in neoplasms (mesothelioma).

The study of the pleural fluid for the presence of flora allows you to identify the causative agent of the disease and identify its antibiotic resistance (sensitivity).

Thoracoscopy is a modern invasive endoscopic method of diagnosis and treatment. Manipulation is carried out under anesthesia lying with the patient side up, the telescope is inserted into the 4.5 intercostal space along the middle axillary line for the most complete examination. The use of a fiberscope allows you to examine the chest cavity, the condition of the lung and pleura, select biopsy specimens (material for research) from all places suspicious for pathological changes. For therapeutic purposes, it is used to evacuate pleural effusion, destroy and cauterize adhesions, pleural lesions in pneumothorax and neoplasms. The advantages compared to open surgery on the organs of the chest cavity include less trauma, less pain, less complications (adhesions), the patient recovers faster after the manipulation.

Thanks to invasive research methods (pleural puncture and thoracoscopy), it is possible to obtain high-quality material for studying and making the correct diagnosis with deciphering the cause of the disease (establishing the etiological factor). Also improve the patient's condition by evacuating the pleural fluid.

Learn more about pleurisy from the video.

Thus, for the diagnosis of pleurisy, it is important to adhere to the stages in the conduct of research. It is necessary to conscientiously carry out a clinical examination of the patient using the skills of a doctor (questionnaire and physical data). The second stage is the use of modern instrumental diagnostic methods available in medicine and their combination with laboratory tests will help diagnose pleurisy, deciphering its cause, which will ensure high-quality treatment and recovery of the patient.

Invasive cardiology

Invasive cardiology

The use of cardiac catheterization for therapeutic purposes gave rise to a whole direction - invasive cardiology. Now with IBS. In patients with acquired and congenital heart defects, instead of open heart surgery, endovascular methods are increasingly being used - balloon coronary angioplasty and balloon valvuloplasty.

The fact that balloon coronary angioplasty is difficult in bifurcation stenoses, calcified, eccentric and complicated plaques (plaques with a thrombus) has led to the appearance of stents. atherectomy and laser angioplasty. They have entered clinical practice since the early 1990s; in many clinics, they already account for 30-40% of endovascular interventions for coronary artery disease. These methods are quite safe and reliable (including long-term results - the risk of re-stenosis), which largely compensates for their higher cost compared to conventional balloon coronary angioplasty.

In the treatment of congenital heart defects in pediatric invasive cardiology, there are a number of radical and palliative endovascular treatments. In addition to balloon valvuloplasty for congenital heart defects, many other methods are used: balloon angioplasty for coarctation of the aorta, balloon angioplasty for stenosis of the branches of the pulmonary artery, embolization of aortopulmonary anastomoses, installation of stents for coarctation of the aorta. stenoses of the branches of the pulmonary artery and after creating a variety of anastomoses, balloon atrial septostomy, closure of the ventricular septal defect. closure of an atrial septal defect and closure of the patent ductus arteriosus.

Cardiology in Israel. Heart treatment in Israel


Diseases of the cardiovascular system are, according to WHO, the leading cause of death and disability in the world. In Israel, where the treatment of heart disease is one of the priority areas of medicine, this figure has been significantly reduced.

To provide effective medical care for CVD, highly qualified doctors are required, as well as the availability of modern equipment at their disposal, which is now equipped with the cardiology departments of the Ichilov clinic.

The level of development of cardiology in Israel makes it possible to save even those patients who were recognized as incurable in other countries, as well as to treat patients from the "risk groups" - those with diabetes. obesity, hyperlipidemia or hypertension.

Diagnosis of heart disease in Israel

To study the cardiovascular system and make an accurate diagnosis, our clinic uses:

  • Ultrasound of the heart (echocardiography, Doppler diagnostics)
  • CT, MRI
  • cardiac catheterization.
  • coronary angiography (performed under local anesthesia)
  • Isotope Scan
  • Invasive hemodynamic monitoring and assessment of pulmonary hypertension
  • Endovasal fiberoptic diagnosis of small vessels.
  • Holter cardiography is a round-the-clock recording of the heart rate using a portable device that does not require the patient to be in the clinic.

The Best-Ichilov Coordination Center will ensure that you get a quick diagnosis without having to wait in long queues, which can be quite long in public hospitals.

Treatment of heart diseases in Israel at the Ichilov clinic

Therapeutic treatment of heart disease in Israel is characterized by a high percentage of effectiveness. Israeli cardiologists skillfully treat coronary disease, heart attack, arrhythmias, angina pectoris, heart failure, including acute and chronic forms, and other diseases of the cardiovascular system.

The cardiology department of the Ichilov hospital consists of a cardio hospital, an intensive care unit, a diagnostic and rehabilitation unit, and subspecialty departments, which employ dozens of world-class doctors and nurses. Among them are professors and teachers of medical faculties, well-known in the world of medical science.

In the Department of Thoracic Surgery of the Ichilov Hospital, cardiosurgical operations of any complexity are performed.

The Best-Ichilov Coordination Center will help you get diagnosed and treated by the best hospital specialists, professors and heads of departments. Among them:

  • Professor Gadi Keren is the head of the cardiology department.
  • Professor Shmul Banai is a specialist in coronary angiography.
  • Professor Sami Wiskin - head. laboratory of electrocardiography and ergometry.
  • Dr. Bella Koifman - head. Department of Diagnostics and Rehabilitation
  • Dr. Dov Wexler - Senior Physician, Diagnostic and Rehabilitation Department

In addition to surgery and drug treatment, water procedures, diets, physiotherapy exercises, cardio equipment, physiotherapy and other methods of treatment and rehabilitation of the heart are used in the cardiology departments of our hospital.

In Ichilov, preventive treatment is also carried out at a high risk of developing cardiovascular diseases.

Invasive cardiology

Invasive methods are used for both diagnostic and therapeutic procedures that require penetration into the body. Among them:

    Stenting for blockage of blood vessels. With the development of atherosclerosis or other problems that lead to narrowing or squeezing of the vessels, a cylindrical frame (stent) made of wire of various metal alloys is inserted into them, which prevents re-narrowing of the vessels and ensures normal blood flow. In addition to the usual ones, special stents impregnated with drugs or stents made of soluble material are used. Which dissolve in about a year when the condition of the vessel returns to normal. Catheterization for replacement or reconstruction of heart valves. This crucial operation today can be performed without opening the chest - a new valve is inserted into the heart through a catheter, under X-ray and echocardiography control. This procedure allows you to help patients who are contraindicated in radical surgery. Probing of vessels - allows not only to perform an examination of the internal cavities of the heart and blood vessels, but also to eliminate blood clots, atherosclerotic plaques, to dissect places of constriction.
  1. Probing of the heart.
  2. Ablations in cardiac arrhythmias.

Ablations are used to block impulses that cause an arrhythmia. To do this, small areas of cardiac tissue are destroyed, and the resulting scar tissue ceases to conduct pathological nerve impulses. Ablation can be carried out by various methods:

    Surgical, when incisions are made on the heart; Heating a section of the heart tissue through an electrode inserted into the body Freezing (cryoablation); Laser ablation; Radiofrequency ablation is the most popular due to the minimal risk of complications and high efficiency.

Ablations of the heart can be performed both during a radical operation and through catheters inserted into the blood vessels. Ablations are especially useful in cases where the patient has contraindications to taking pharmaceuticals.

Every year, Israel, including our clinic, is visited by tens of thousands of patients from other countries who want to receive high-quality and professional treatment from our doctors. If you need the help of Israeli cardiologists or cardiac surgeons, you can contact the specialists of the Best-Ichilov coordination center using any contact that is convenient for you from those listed on the site.

Cardiac surgery in Israel: invasive cardiology


Invasive cardiology - beauty and beauty and pride of Israeli medicine. The spectrum of diseases of the heart and blood vessels that can be treated by endovascular methods through blood vessels is quite wide.

It is customary to refer to invasive cardiology diseases of the cardiovascular system. requiring the intervention of a cardiac surgeon who owns endovascular methods of treatment. They are understood as surgical manipulations performed on blood vessels using visualization tools (X-ray, computed tomography, etc.) and special surgical instruments.

Invasive cardiology in Israel is one of the most successful directions of the famous Israeli medicine both in the field of diagnostics and treatment of diseases of the heart and blood vessels.

One of the main areas of application of invasive cardiology is angiography or its special case - aortography. We are talking about the study of the state of blood vessels or specifically the aorta. A radioactive contrast agent is injected into the coronary arteries. Tracking its distribution in the circulatory network surrounding the heart using X-ray, the cardiac surgeon sees the structure and, accordingly, disturbances in the work of the heart, its vessels and valves, determines the presence of blood clots and cholesterol plaques. Directly during the study, the surgeon can perform invasive procedures to restore blood flow.

Stenosis of the coronary arteries, detected with the help of coronary angiography, is corrected with the help of stenting - the installation of a stent, which ensures the maintenance of the lumen of the vessels and the normal level of blood flow in them. In the case when a lesion of three or more coronary vessels is diagnosed, a cardiac surgeon may prescribe a major cardiac surgery to perform bypass surgery - artificial blood flow bypassing the blocked areas of the coronary network.

Methods invasive cardiology in Israel treatment of some heart defects in children and adults, such as valvular stenosis or some defects of the interatrial and interventricular septum, bifurcation arterial stenosis, as well as the treatment of coronary heart disease, heart attacks and strokes in an acute state, postinfarction cardiosclerosis, unstable angina pectoris, chronic occlusions etc.

The following is a partial list of the main methods of invasive cardiology:

  • heart valve replacement
  • catheterization and expansion of the main vessels and heart
  • aortic prosthesis
  • vascular stenting. carotid artery, coronary vessels, bifurcation stenoses, pulmonary arteries, as well as stenting and angioplasty of the iliac and femoral arteries.
  • implantation of permanent pacemakers
  • embolization of cerebral aneurysms

Modern stents, widely used by Israeli cardiac surgeons, have special coatings that prevent recurrent vascular stenosis. The substances that make up the coating have antitumor, wound healing, and immunosuppressive effects, which makes it possible to achieve good engraftment of stents without a rejection reaction and other side effects.

As a rule, endovascular operations are performed under local anesthesia, they have a minimal risk of bleeding and do not cause infection of the surgical wound. Invasive cardiology is characterized by rapid recovery and an almost complete absence of side effects.

Israeli medicine is rightly proud of its staff - world-famous specialists who work in conditions of brilliant technical equipment.

Anyone who is interested in treatment should contact the consultant of the Medserver portal. You are guaranteed comprehensive assistance in organizing treatment in Israel with leading doctors, at a convenient time and at reasonable prices.

The center of invasive cardiology in Simferopol will be renovated and equipped with the latest equipment

Minimally invasive surgeries

Hemorrhoids are a rather unpleasant thing that can happen to anyone. This disease can affect anyone, regardless of gender or age. It is important to remember that the sooner you turn to a specialist for help, the sooner you can recover from this unpleasant disease without resorting to surgical intervention. In the initial stages of the disease, when the formation of external hemorrhoids has not yet occurred, it is much easier to cope with the disease.


Hemorrhoids, the treatment of which should be carried out under the supervision of a specialist, provides for two methods of treatment - conservative (includes the use of ointments, suppositories, physical education, following a special diet) and radical, which involves getting rid of hemorrhoids depending on the stage of the disease. The latter, in turn, is divided into minimally invasive and surgical intervention. This article will focus on minimally invasive methods of treating hemorrhoids.

Minimally invasive options for the treatment of hemorrhoids involve minimal surgical intervention with the maximum level of comfort for the patient. This combination is achieved through the use of the latest medical technologies.

  • Sclerosis of hemorrhoids is performed using a special needle, through which a special substance is introduced into the hemorrhoids, leading to wrinkling and reduction of the nodes.
  • Photocoagulation. This method is used at the initial stage of the disease, and consists in the effect of a laser on the hemorrhoidal tissue. It literally “evaporates” the tissue of the hemorrhoid, as a result of which the disease can be crushed at the very beginning.
  • Cryodestruction consists in the effect of extremely low temperatures on the tissue of the hemorrhoid, as a result, the inflamed areas are frozen and rejected. This method shows quite good results in the first and second stages of hemorrhoids, when the formation of external hemorrhoids has not yet occurred.
  • vacuum ligation. In this case, the node itself is sucked into a special apparatus using a vacuum tube, and a special latex ring is applied to the base of the leg that feeds the node. After a while, tissue necrosis and rejection of the hemorrhoid occurs. This method is also effective in the initial stages, when the formation of external hemorrhoids has not yet occurred.

There are two more methods of mini-invasive treatment of hemorrhoids - the TND method (carried out under the control of a special ultrasonic sensor, and consists in flashing the vessels above the node, which nourish the tissues and contribute to the growth of the hemorrhoid) and the Longo method (in this case, the mucous membrane, which is located above the node , incised). Both of these methods are aimed at stopping the blood supply and nutrition of the hemorrhoid. However, as practice shows, the node can also be fed from other sources, therefore, with such treatment options, relapses of the disease are possible. That is why these methods have not found wide application in modern medicine and are gradually disappearing into history.

As you can see, at the initial stages of the development of the disease, it can be defeated without surgical intervention. Minimally invasive methods of treating hemorrhoids are not suitable for all patients, so before proceeding with radical treatment, you should consult a specialist.

Invasive methods for examining the heart

Invasive research methods - probing and puncture of the heart cavities, angiocardiography - are widely used in cardiology and other branches of medicine, due to the need to establish anatomical and functional diagnosis of heart disease and its consequences. These data are necessary for the selection of rational methods of treatment and evaluation of their effectiveness. Currently, the diagnosis of most congenital and acquired malformations, heart tumors, diseases of the myocardium and great vessels cannot be considered reliable without confirmation by data from invasive research methods. In the absence of data from these studies, it is difficult to solve the fundamental issues of treatment tactics.

Cardiac catheterization has been developed and widely used in connection with the surgical treatment of congenital and acquired heart defects. It is used to measure pressure in the chambers of the heart, pulmonary artery, to determine the amount of blood discharge through pathological shunts, cardiac output in order to clarify the nature of the defect and its severity, record intracardiac electro- and phonocardiograms, perform contrast studies of the heart and blood vessels.

Most often, special research methods are used for combined heart defects to clarify the degree of stenosis and insufficiency, combined defects to identify concomitant pathology, for aortic valve defects to determine their severity, as well as to monitor the effectiveness of medical and surgical treatment.

Invasive methods are not advisable to use with a low severity of defects and hemodynamic disorders, the absence of hypertrophy of the heart (according to ECG data) and small deviations from the norm in the size and configuration of the heart, according to x-ray data.
The risk of using invasive research methods is increased in patients with heart defects in the stage of severe respiratory and circulatory failure. With a positive iodine test and acute intercurrent diseases, studies are contraindicated.

For a contrast study of blood vessels, heart cavities and pathological communications between them, 60-76% solutions of verografin or urotrast are used in an amount of 5-40 ml, which are injected using an automatic syringe at a rate of 25-30 ml / s. On the x-ray, a clear image of the heart and blood vessels is obtained.

In children of preschool and primary school age, examinations are carried out under anesthesia; in children of senior school age and adolescents, under local anesthesia.

Invasive research methods are currently quite well mastered, safe, highly informative and therefore are used quite often for diagnostic and therapeutic purposes due to the need for pathophysiological studies of intracardiac, pulmonary and systemic hemodynamics, as well as to monitor the effectiveness of treatment. Simultaneously with the clarification of the diagnosis of the defect, the degree of its severity and consequences, the minute volume of the large and small circles of blood circulation, the pressure in the pulmonary artery and in the chambers of the heart, as well as the change in these indicators after medical and surgical treatment, are determined.

Catheterization of the right heart is performed to diagnose defects of the right atrioventricular valve, pulmonary valve, differential diagnosis of acquired and congenital defects, to determine the degree of pulmonary hypertension.

Under local anesthesia, from a small skin incision (1-2 cm), the medial saphenous vein of the left arm is exposed in the elbow bend or in the shoulder area, it is tied up proximal to the ligature, the lumen is opened with a transverse incision, and a special catheter filled with isotonic sodium chloride solution with heparin is inserted. The solution is administered drip, continuously, which prevents spasm of the vein, thrombosis of the catheter, which, under the control of the X-ray machine, is passed through the innominate and superior vena cava, the right atrium and the ventricle into the pulmonary artery until wedging in one of its branches. Record the pressure curve in the pulmonary capillaries, take blood to determine the gas composition. After that, the catheter is removed under the control of the X-ray machine. At the same time, pressure is measured and blood is taken sequentially from the pulmonary artery, right ventricle, right atrium and vena cava to determine its oxygen saturation.

If it is necessary to conduct a contrast study through the same catheter, a contrast agent is injected into any part of the pulmonary artery or heart chamber in accordance with the task of the study.

Then the catheter is removed, the vein is ligated, and the skin is sutured with 1-2 interrupted sutures.

Probing of the left atrium (transseptal puncture of the left atrium according to Manfredi and Ross using the Seldinger technique) is performed to diagnose defects of the left atrioventricular valve and diseases of the left atrium (thrombosis, tumors).

Under local anesthesia with a special needle with a diameter of 1.5-2 mm, the right femoral vein is punctured. A special metal conductor is inserted through the needle into the lumen of the vein at a distance of 10-15 cm. Then the needle is removed and a catheter is inserted into the vein along the conductor, as if along a rod, after which the conductor is removed.

Under the control of the x-ray machine, the catheter is placed in the right atrium in the region of the oval fossa. After that, a special long mandrin-needle with a pointed end is inserted into it, which in the initial position for puncture of the interatrial septum protrudes 1 cm from the catheter. In the direction of the inside and back at an angle of 45 ° (with the patient in a horizontal position on the back), the interatrial septum is punctured and the catheter is advanced, and the mandrin is pulled back and removed. The position of the catheter in the left atrium is monitored and confirmed by recording the pressure curve and the degree of blood oxygen saturation. If necessary, the catheter can be passed into the cavity of the left ventricle, the pressure in it and the pressure gradient on the left atrioventricular valve can be measured, and contrast can be performed in the left cavities of the heart and aorta.

Transthoracic puncture of the left atrium according to Bjork in the area of ​​the angle of the right scapula was widely used in the 60-70s. Now, due to frequently occurring complications (pneumothorax, hemoptysis, pulmonary edema, hemopericardium), this method is not used.

Retrograde catheterization of the aorta and left ventricle (according to Seldinger) is used to diagnose diseases and malformations of the aorta, malformations of the aortic and left atrioventricular valves, as well as for the purpose of differential diagnosis of malformations of the left atrioventricular valve and defects of the heart septum.

One of the femoral arteries is used to pass the catheter into the aorta. During the study, the patient lies on his back. The skin in the groin area is treated with an antiseptic. At the site of the proposed puncture, anesthesia is performed with a solution of novocaine. The skin is incised with an eye scalpel at the site of the needle and catheter, and the femoral artery is punctured at an angle of 45 °. After removing the mandrin from the needle, scarlet blood is poured out under pressure, which confirms the presence of the needle in the lumen of the artery. Through the needle, a special mandrel guide is inserted into it at a distance of 10-15 cm, the needle is removed, and the place where the guide is inserted into the artery is pressed with a finger. A catheter is strung on the conductor, which is inserted along the conductor through the channel formed by the needle into the lumen of the femoral artery, and then, under the control of the X-ray apparatus, together with the conductor, retrograde against blood flow into the ascending aorta.

The main dangers and complications of invasive research methods. The most frequent and transient complications are heart rhythm disturbances in the form of atrial and ventricular extrasystoles, tachyarrhythmias.

Bundle branch block, atrioventricular block, atrial fibrillation, and ventricular fibrillation are rare. If the research technique is violated, damage to the walls of the heart and blood vessels, hemopericardium, probe nodulation in the heart cavities, thrombosis and embolism of the pulmonary artery, and pulmonary edema are sometimes observed.

With a methodically correctly performed study, constant monitoring of the patient during the procedure, it is possible to prevent and promptly eliminate the complications that have arisen.

Data of special research methods in the diagnosis and assessment of the severity of congenital heart and vascular diseases. The main methods for diagnosing congenital heart defects are auscultation, phonocardiography, electrocardiography, echocardiography, fluoroscopy, and radiography of the heart and pulmonary vessels. Important, often mandatory research methods are probing the cavities of the heart and blood vessels, puncture of the heart, angiocardiography, rheography and radiocirculography. Ballistocardiography, sphygmography, tachooscillography in the diagnosis of heart defects are not very informative and their importance should not be exaggerated. The variety of congenital heart defects consists of several anatomical variants, among which narrowing of the left and right atrioventricular orifices and vascular orifices (stenosis), pathological communication between the chambers of the heart and blood vessels (defect) and a combination of stenosis with a defect can be distinguished. In these cases, characteristic pathophysiological disorders, mechanisms of compensation and decompensation are observed.

With the help of invasive research methods, it is possible to clarify the anatomical features of the defect, identify pathophysiological disorders and functional reserves, determine the location and nature of the narrowing with the calculation of the pressure gradient, the size of the heart chambers, and the diameter of the vessels. These data make it possible to establish the severity and severity of heart disease, the possibility and options for surgical treatment, the mode of behavior and physical activity, if surgical treatment is not used.

Probing and angiocardiography are necessary for stenosis of the aortic orifice, pulmonary artery, right and left atrioventricular orifices, coarctation of the aorta, Fallot group defects, Ebstein anomaly and other anomalies, when questions about indications for surgical methods of treatment are being resolved.

With an isolated narrowing of the lumen of the vessels, the severity of the disease is determined by the magnitude of the blood pressure in the ventricles. In this case, the pressure in the pulmonary artery or aorta is maintained at the level of normal or subnormal values. So, for example, with narrowing of the mouth of the pulmonary artery and an increase in systolic pressure in the right ventricle to 8.0 kPa (60 mm Hg), stage I of the defect is diagnosed. In this case, surgical treatment can be refrained from.

At II and III stages of the defect and systolic pressure from 8.0 to 13.3 kPa (from 60 to 100 mm Hg) and more, surgical treatment is absolutely indicated.

Angiocardiography makes it possible to determine the place of narrowing - subvalvular, valvular, supravalvular, anatomical features of the output section of the ventricle and the excretory vessel.

This makes it possible to choose the optimal variant of surgical intervention.

With a systolic pressure in the ventricle above 40.0 kPa (300 mm Hg), a pronounced concentric hypertrophy of the heart muscle is observed with a decrease in the ventricular cavity to 20-30 ml. At the same time, diastolic pressure in the ventricle increases, the minute volume of the heart decreases, and small ventricular syndrome develops. Severe dystrophic changes in the hypertrophied myocardium, coronary blood flow deficiency, low functional reserves limit the physical activity of patients and the possibilities of surgical treatment.

In case of heart defects with the presence of pathological messages between the chambers, sounding and angiocardiography data make it possible to establish the localization of a septal defect. The place of the pathological message is determined by detecting the discharge of arterial blood, the change in the oxygen capacity of the blood in the chambers, the flow of a contrast agent from the high pressure chamber into the low pressure chamber. The same information can be obtained by thermodilution and contrast echocardiography.

For example, the oxygen capacity of the blood of the right atrium was 14 vol. %, and the right ventricle - 16 vol. %, blood oxygen saturation, respectively - 75 and 85%. In the presence of such data, it can be concluded that at the level of the ventricles there is a discharge of blood from left to right through a septal defect. The difference in the oxygen capacity of the blood in the chambers of the heart is up to 1 vol. % is considered unreliable and therefore, in the absence of other data, a diagnosis of heart disease cannot be made.

Absolutely reliable for the diagnosis of pathological communication between the chambers and vessels can be the passage of a radiopaque probe through the defect, followed by an analysis of the position of the catheter, blood and pressure.

Based on the sounding data and the Fick principle, it is possible to calculate the resistance of the vessels of the large and small circles, the work of the left and right ventricles, their performance. With septal defects, as a rule, hypervolemia of the pulmonary circulation is observed, the minute volume of which can be 15-20 l / min or more.

With septal defects, as with stenosis, it is imperative to measure the pressure in the chambers of the heart and the main vessels, determine the degree of pulmonary hypertension, which is a frequent and characteristic complication of defects in this group.

Special research methods in the diagnosis and evaluation of acquired heart defects in children. Puncture and probing of the heart, aortocardiography are used to clarify the degree of stenosis and insufficiency in combined defect, differential diagnosis of acquired and congenital defects, study of pathophysiological changes in intracardiac and pulmonary hemodynamics.

It is known that the left atrioventricular valve is affected most often. One of the early hemodynamic signs of malformation is an increase in pressure in the left atrium. At the same time, characteristic changes in the configuration of the curve are revealed, which have a differential diagnostic value.

The left atrial blood pressure curve consists of two positive waves and two negative ones.

The level of pressure in the left atrium and the magnitude of the diastolic pressure gradient between the left atrium and the left ventricle can be used to judge the degree of narrowing of the left atrioventricular orifice. Normally, the pressure difference is 0.1-0.3 kPa (1-2 mm Hg), and with stenosis it can reach 2.7-4.0 kPa (20-30 mm Hg). However, there is no linear relationship between the degree of narrowing and the magnitude of the diastolic pressure gradient. This indicator is influenced, in addition to a decrease in the area of ​​the left atrioventricular orifice, by the contractile function of the myocardium, changes in rhythm and cardiac output, and the presence of concomitant mitral insufficiency stenosis.

It should also be noted the possibility, according to the sounding of the pulmonary artery and the level of pulmonary capillary pressure, to determine the area of ​​the left atrioventricular orifice according to the Gorlinykh formula.

When comparing the size of the area of ​​the left atrioventricular orifice, calculated according to the Gorlinykh formula, with the area measured during the operation, the high information content and value of this method are confirmed.

Ventriculo- and angiography provide important data for the differential diagnosis of defects and their severity when deciding on surgical treatment.

Most often they have to be used in children with the differentiation of mitral insufficiency and ventricular septal defect. The flow of a contrast agent during systole from the left ventricle into the left atrium indicates mitral insufficiency. If the contrast agent enters the right ventricle, then there is a ventricular septal defect.

By the amount of incoming contrast agent from one chamber or vessel to another chamber, the time of its retention in the chamber and the expansion of the cavities, the severity of the defect is determined.

Special methods for studying the cardiovascular system - probing, radiocirculography are widely used to determine the consequences of a defect and the functional reserves of the heart. All children with stage III and IV mitral stenosis and many children with mitral regurgitation have pulmonary hypertension. Often, systolic pressure in the pulmonary artery is very high and reaches 13.3-18.7 kPa, or 100-140 mm Hg. Art.

During the study of cardiac output, it turned out that even with severe heart defects, but without circulatory decompensation of IIB-III degree, there are no deviations from normal values.

Therefore, at the present stage, only with the use of invasive methods (according to indications), the diagnosis and treatment of congenital and acquired heart defects and some other diseases of the cardiovascular system can be optimal.

Sufficient technical equipment of the leading medical institutions, the safety of studies that have been mastered and tested by practice, allow surgeons to use them in accordance with the developed indications.

It should be noted that X-ray endovascular surgery has emerged and continues to develop on the basis of invasive research methods. This is a new direction in the treatment of defects and diseases of the heart and blood vessels. With the help of special probes and devices that are introduced into the vascular bed, it is possible to improve the blood circulation of organs. Creation or expansion of an atrial septal defect, dilatation of narrowing of the pulmonary and renal arteries and aorta facilitate the course of defects. The introduction of special microspheres and spirals through the probes allows you to stop bleeding, eliminate or reduce the tumor. Endovascular surgery can be used independently, as well as in combination with other methods of treating sick children.

INVASIVE METHODS OF PRENATAL DIAGNOSIS

Prenatal (in other words, prenatal) diagnostics is one of the youngest and most rapidly developing areas of modern reproductive medicine. Representing the process of detecting or excluding various diseases in the fetus located in the uterus, prenatal diagnosis and genetic counseling based on its results answer vital questions for every future parent. Is the fetus sick or not? How can the detected disease affect the quality of life of the unborn child? Is it possible to effectively treat the disease after the birth of a baby? These answers allow the family to consciously and timely resolve the issue of the future fate of pregnancy - and thereby alleviate the mental trauma caused by the birth of a baby with an incurable, disabling pathology.

Modern prenatal diagnosis uses a variety of technologies. All of them have different capabilities and degrees of reliability. Some of these technologies - ultrasound screening (dynamic observation) of fetal development and screening of maternal serum factors are considered non-invasive or minimally invasive - i.e. do not provide for surgical intervention in the uterine cavity. Practically safe for the fetus, these diagnostic procedures are recommended for all expectant mothers without exception. Other technologies (chorionic biopsy or amniocentesis, for example) are invasive - i.e. suggest a surgical invasion of the uterine cavity in order to take the fetal material for subsequent laboratory testing.

It is clear that invasive procedures are not safe for the fetus and therefore are practiced only in special cases. Within the framework of one article, it is impossible to analyze in detail all the situations in which a family may need invasive diagnostic procedures - the manifestations of hereditary and congenital diseases known to modern medicine are too diverse. However, a general recommendation to all families planning the birth of a child can still be given: be sure to visit a medical genetic consultation (preferably even before pregnancy) and in no case ignore ultrasound and serum screening. This will make it possible to timely resolve the issue of the need (and justification) for an invasive study. With the main characteristics of various methods prenatal diagnosis can be found in the tables below.

AMNIOCENTESIS

The vast majority of the methods listed below prenatal diagnosis congenital and hereditary diseases today is widely practiced in Russia. Ultrasound screening of pregnant women is carried out in antenatal clinics or medical genetic services.

In the same place (in a number of cities), screening of maternal serum factors (the so-called "triple test") can also be done. Invasive procedures are carried out mainly in large obstetric centers or interregional (regional) medical genetic consultations. Perhaps in the very near future all these types of diagnostic assistance in Russia will be concentrated in special centers prenatal diagnosis. At least, this is how the Ministry of Health of the Russian Federation sees the solution to the problem.

CHORION

Well, as they say, wait and see. In the meantime, it would not hurt for all residents of cities and villages of the fatherland planning to replenish the family to ask in advance what opportunities in the region prenatal diagnosis has local medicine. And if these opportunities are insufficient, and the need for quality prenatal diagnosis objectively available, you should immediately focus on the examination of the expectant mother outside the native locality.

CARDOCENTESIS

Moreover, part of the financial costs in this case may well be borne by the very local health care, in the arsenal of which there is no type of diagnostic service necessary for the family.

INVASIVE METHODS OF PRENATAL DIAGNOSIS

Method name

Terms of pregnancy

Indications for carrying out

Object of study

Methodology

Method capabilities

Advantages of the method

Disadvantages of the method, risk during the procedure

Chorionic biopsy

10-11 weeks.

High probability of hereditary diseases (the probability of detecting a serious illness in the fetus, comparable to the risk of miscarriage after a biopsy).

Chorionic cells (outer germinal membrane).

1 way. A small amount of chorionic tissue is aspirated with a syringe through a catheter inserted into the cervical canal. 2 way. A tissue sample is aspirated into a syringe using a long needle inserted into the uterine cavity through the abdominal wall. Both options for chorion biopsy are performed on an outpatient basis or with a short-term hospitalization of a pregnant woman. Manipulation is performed under ultrasound control. Depending on the practice adopted in a particular medical institution, a biopsy is performed either under local or general anesthesia (anesthesia). Before the procedure, a woman needs to undergo a laboratory examination (blood tests, smears, etc.).

Definition in the fetus of Down syndrome, Edwards syndrome, Patau and other chromosomal diseases, accompanied by gross deformities or mental retardation. Diagnosis of genetic diseases (the range of diagnosed hereditary diseases depends on the capabilities of a particular laboratory and can vary from single genetic syndromes to dozens of different disabling diseases). Determination of the sex of the fetus. Establishment of biological relationship (paternity).

Quick results (within 3-4 days after taking the material). It is possible to diagnose a severe disabling disease in a fetus in the period up to the 12th week, when abortion occurs with fewer complications for a woman, and the stress load on family members also decreases.

For a number of technical reasons, it is not always possible to conduct a qualitative analysis of tissue samples. There is a small risk of false positive and false negative results due to the phenomenon of so-called. "placental mosaicism" (non-identity of the genome of chorion and embryo cells). Prolonged exposure of the fetus to ultrasound, the harmlessness of which has not been proven. Risk of accidental damage to the amniotic sac. The risk of adverse effects on the course of pregnancy in Rh-conflict. Risk of miscarriage (from 2 to 6% depending on the condition of the woman). The risk of infection of the fetus (1-2%). The risk of bleeding in a woman (1-2%). Risk (less than 1%) of some abnormalities in the development of the fetus: cases of gross deformities of the limbs in newborns undergoing chorionic biopsy have been described. In general, the risk of complications from chorionic biopsy is low (less than 2%).

Placentocentesis (late chorion biopsy)

II trimester of pregnancy.

Similar indications for a chorionic biopsy.

Cells of the placenta.

Similar to the method of the 2nd method of chorion biopsy described above. It is performed under local or general anesthesia, on an outpatient basis or with a short-term hospitalization of a woman. The requirements for examining a pregnant woman before placentocentesis are identical to those for a chorionic biopsy.

Similar to the possibilities of chorion biopsy.

The cultivation of cells obtained during placentocentesis may be less effective than the cultivation of chorion cells, so sometimes (very rarely) there is a need to repeat the procedure. This risk is absent in laboratories practicing modern methods of cytogenetic diagnostics. Conducting an examination at a sufficiently long gestational age (in case of detection of a serious pathology, termination of pregnancy during this period requires a long hospitalization and is fraught with complications).

Amniocentesis

15-16 weeks.

The same as with chorionic biopsy and placentocentesis. Suspicion of the presence of certain congenital diseases and pathological conditions in the fetus.

Amniotic fluid and fetal cells in it (desquamated fetal skin cells, epitheliocytes from the urinary tract, etc.).

Amniotic fluid is drawn into the syringe with a needle inserted into the uterine cavity through the abdominal wall. Manipulation is performed under the control of an ultrasound machine, on an outpatient basis or with short-term hospitalization. Local anesthesia is most often used, but it is quite possible to carry out the procedure under general anesthesia. Before the procedure, a pregnant woman undergoes a laboratory examination similar to that of a chorionic biopsy and placentocentesis.

Diagnosis of various chromosomal and gene diseases. Determination of the degree of maturity of the lungs of the fetus. Determination of the degree of oxygen starvation of the fetus. Determining the severity of the Rh conflict between mother and fetus. Diagnosis of some fetal malformations (for example, gross deformities of the brain and spinal cord anencephaly, exencephaly, spinal hernia, etc.).

Wider (compared to chorion biopsy and other invasive methods of prenatal diagnosis) range of detected pathologies. The risk of miscarriage is somewhat less than with a chorionic biopsy. This risk is only 0.5-1% higher than in pregnant women who did not undergo invasive examinations at all.

Technological problems. Since there are very few fetal cells in the collected sample, it is necessary to give them the opportunity to multiply in artificial conditions. This requires special nutrient media, a certain temperature, reagents, sophisticated equipment. Quite a long time (from 2 to 6 weeks) for the analysis of chromosomes. Results are obtained on average by 20-22 weeks. When the diagnosis is confirmed, termination of pregnancy at this time is accompanied by a large number of complications than, for example, at the 12th week. Stronger and moral trauma of family members 1 . Prolonged exposure of the fetus to ultrasound, the harmlessness of which has not been proven. The risk of having a small child is slightly increased. There is a low (less than 1%) risk of respiratory distress in the newborn.

Cordocentesis

After the 18th week of pregnancy.

Similar to those for chorionic biopsy and placentocentesis.

Cord blood of the fetus.

A fetal blood sample is obtained from the umbilical cord vein, which is punctured under ultrasound control with a needle inserted into the uterine cavity through a puncture in the anterior abdominal wall of the woman. The procedure is performed under local or general anesthesia, on an outpatient basis or with a short-term hospitalization of a woman. The requirements for examining a woman before cordocentesis are identical to those for a chorionic biopsy.

Similar to the possibilities of chorionic biopsy and placentocentesis, partially amniocentesis. The possibility of therapeutic manipulations (administration of drugs, etc.).

Minimal chance of complications.

Conducting a survey at a long gestational age (in case of detection of a serious pathology, termination of pregnancy during this period requires a long hospitalization and is fraught with complications).

Fetal tissue biopsy as a diagnostic procedure carried out in the second trimester of pregnancy under ultrasound control. To diagnose severe skin lesions (ichthyosis, epidermolysis), a biopsy of the fetal skin is taken, followed by a pathomorphological study. Fetal muscle biopsy is performed to diagnose Duchenne dystrophy. The biopsy is examined by the immunofluorescence method. Such methods, in these and in a number of other cases, give more correct results, which allows you to make an accurate diagnosis or confidently reject it.

Fetoscopy(probe insertion and examination of the fetus) with modern flexible optical technology does not present great difficulties. However, the method of visual examination of the fetus to detect congenital malformations is used only for special indications. It is carried out at the 18-19th week of pregnancy. Fetoscopy requires insertion of an endoscope into the amniotic cavity, which can cause pregnancy complications. Miscarriages occur in 7-8% of cases. At the same time, almost all congenital malformations that can be seen with the help of fetoscopy are also diagnosed with the help of ultrasound. It is clear that the ultrasound procedure is simpler and safer.

Literary References:

    http://www.medichelp.ru/posts/view/5863

    http://www.9months.ru/press/10/13/index.shtml

    Obstetrics. National leadership, Ed. E.K. Ailamazyan, V.I. Kulakova, V.E. Radzinsky, G.M. Savelyeva.

    All about pregnancy and childbirth S. Zaitsev

    Obstetrics and gynecology A brief guide to practical skills Kostyuchek D.F.